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| Place of Origin: | China |
| Brand Name: | Youngshe |
| Certification: | / |
| Model Number: | high quality |
| Minimum Order Quantity: | 1g |
|---|---|
| Price: | USD50-5000/g |
| Packaging Details: | plastic bottle,1g/bottle,5g/bottle,10g/bottle, or according to the customer's request. |
| Delivery Time: | 3-5 working days |
| Payment Terms: | L/C, T/T, Western Union, MoneyGram |
| Supply Ability: | 300g/month |
| Shelf Life: | Two Years | Color: | White |
|---|---|---|---|
| Storage Condition: : | -20 OC |
Basic information:
| Products Name |
Recombinant Bovine Enterokinase ( rb-EK)
|
| Catalog Number: | YSRBEK |
| Formulation | Lyophilized from 50 mM Tris-HCl, pH8.0 |
| Mol. Wt | 26.2 kDa, 30 kDa by SDS-PAGE analysis |
| Theoretical pI | 5.20 |
| Resources | Escherichia coli (E. coli) |
| Purity | ≥95% by SDS-PAGE analysis |
| Storage | 2-8℃,Cool and dry place, Keep away from strong sunlight |
| Remark | In case of quality issues, please contact us within 15days after received of products. |
Description:
Enterokinase is a serine protease that can specifically cleave the sequence Asp-Asp-Asp-Asp-Lys after the lysine residue . It applies for digesting fusion proteins to release the fusion partner or tag from the target protein.
Native enterokinase is composed of a 115 kDa heavy chain as a membrane anchor and a 35 kDa light chain as the catalytic subunit. Recombinant bovine enterokinase is a specially prepared recombinant enzyme, consisting of the catalytic subunit of the holoenzyme. This subunit is expressed and purified from E.coli, yielding an enzyme with higher specific activity. This results in more
efficient cleavage using less enzyme.
Note:
1. Rb-EK is known to be inhibited by >2 M Urea, >250 mM NaCl, >20 mM β-ME, >0.1% SDS, or >50 mM imidazole. Dialyze your fusion protein to remove the inhibitors prior to digestion with rb-EK.
2. Avoid using phosphate buffer.
We are a professional Peptide Manufacturer in China. We can do :
1. Custom peptide synthesis ( long peptide sequence up to 200AA)
3. Cosmetic Peptide (More than 100 kinds in stock)
4. Catalog Peptide (More than 3000 kinds in stock)
5. Botanical monomer (2000 kinds available, 98% pure)
Our Advantages: quality stable, good Price and fast delivery
Report: HPLC/ Mass/ COA/ NMR .
